FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6800200
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT73720.10840857621834653No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT70440.10358518867092145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11150.024.7983444
GTATGCC12200.022.66405745
TATGCCG13100.021.37400246
GCACACG14250.021.3672811
CTAGCAG27950.020.1596288
ACGTCTG15650.020.12701415
CTGACTC31450.018.5889822
TCGTATG15500.018.2904243
CTCGTAT15900.017.38990442
GCTGACT34800.017.1055931
TAGCAGA37200.015.8053749
CACGTCT19700.015.63379714
CGGGCGC11900.015.58763115
GGCGGGC11950.015.52229813
CACACGT20100.015.49668312
AGCACAC20850.015.44281910
GACTCTA41250.015.2753934
ACACGTC20250.015.20917513
ACTCTAG39800.014.7765565
CTATCGA41000.014.2556517
Produced by FastQC (version 0.11.5)