FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8813499
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17650.032.3227744
TATGCCG17650.031.33127246
GTATGCC19000.029.65773245
GCACACG24450.024.33469211
ACGTCTG24350.024.2906215
TCGTATG24900.023.3334743
CACACGT28050.021.21152312
CTCGTAT25300.021.02779242
CACGTCT28850.020.86572614
CACAGTG28000.020.74917833
AGCACAC30300.019.98293510
CACACAG28850.019.8953431
ACACGTC30500.019.62239813
ACACAGT29300.019.35076932
GCCGTCT32000.017.7182549
CAGTCAC32600.017.39254227
CTAGCAG28050.017.3433428
CCAGTCA33100.017.12981426
GAGCACA37850.016.5515259
TCCAGTC35650.016.19943625
Produced by FastQC (version 0.11.5)