FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219367
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG120640.37473205136289217No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC75170.23349310594287634No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG63430.19702630983047287No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT57680.1791656558571918No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC51200.15903747537947677No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG48840.151706841748704No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC48470.15055754749303202No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG46040.14300947981388887No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG46030.1429784178069788No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA41810.129870250890936No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC37210.11558172771231115No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA36200.1124444650143957No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC35690.11086030266198293No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT35200.10933826432339028No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC32990.10247356079626833No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA32600.10126214252677622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC4350.025.7482145
CTAGCAG15500.024.6112638
CGTATGC4450.024.38304944
TATGCCG4500.024.1121346
CTGACTC17000.023.4737662
GTGCGCA37900.023.45854868
CCGGGGT2550.023.341931
TGCGCAT38200.023.2746869
GCTGACT17000.022.6554051
GGTGCGC41050.022.084167
AGGTGCG40950.021.79612766
GACTCTA20300.020.8648934
ACTCTAG21800.020.553245
GCACACG6100.019.50689711
CGCGGGG1801.6986269E-719.451611
AAGGTGC47450.019.25325465
GTCACGC6000.019.24961529
TCGTATG6050.019.09171343
CTCGTAT5900.018.98326142
TAGCAGA20700.017.9212329
Produced by FastQC (version 0.11.5)