FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_047.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_047.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6952701
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG263440.37890310542622213No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC172130.24757284974573193No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT140580.2021948017036832No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG138500.1992031585998017No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC113890.16380684283704997No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG108170.15557982430137582No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC107550.15468808453002653No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG105570.15184027042152395No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG105410.15161014402891768No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA98520.14170032624731022No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC80080.11517825949943769No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC76320.10977028927319037No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT75610.10874910340600005No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA75060.107958043931416No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG74270.10682179486792255No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA74010.10644783947993737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG36350.029.2693528
CTGACTC37100.028.8696462
CGTATGC8950.026.59280844
TATGCCG9250.026.10853846
GTATGCC10150.024.48316245
GCTGACT44200.024.2362211
GCACACG12000.023.6236911
ACTCTAG54700.022.3319055
ACGTCTG12300.021.9096715
GACTCTA52300.021.8843544
GTGCGCA88150.021.87878668
TAGCAGA49050.021.7622999
TGCGCAT89450.021.56066169
ACTGACC11650.020.72861332
AGGTGCG94800.020.45436566
CACACGT13100.020.30436312
GGTGCGC97950.020.01082667
CGCGGGG4200.020.0045031
CTCTAGC55050.019.3924776
TCTATCG56250.019.10143916
Produced by FastQC (version 0.11.5)