FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8907699
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG222150.24939100434354594No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC165020.18525547394450576No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT141260.15858191885468964No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG117020.13136950406608935No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC110020.1235111334588203TruSeq Adapter, Index 3 (100% over 50bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG97360.10929870890338796No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC97340.10927625641593862No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC94470.1060543244669583No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA91220.10240579525644053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21900.039.7945344
TATGCCG22800.037.91667646
GTATGCC23650.037.4418745
CACTTAG24400.037.00758431
GCACACG24950.036.61220611
CTCGTAT24900.034.297242
ACGTCTG26500.034.20640615
TAGGCAT25900.033.37726235
TTAGGCA27250.032.87963534
CACACGT28000.032.873912
TCGTATG26950.032.72746343
ATGCCGT26850.032.4581147
AGCACAC30800.030.2264310
TCACTTA30300.029.80148530
CACGTCT30950.029.40127814
ACACGTC32950.027.82911513
GCCGTCT31800.027.51541949
CTAGCAG40400.026.6824258
CATCTCG31800.026.41480339
GGCATCT32200.026.19492137