Basic Statistics
Measure | Value |
---|---|
Filename | HFKN2BGX9_n01_NCR6_046.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8907699 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG | 22215 | 0.24939100434354594 | No Hit |
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC | 16502 | 0.18525547394450576 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT | 14126 | 0.15858191885468964 | No Hit |
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG | 11702 | 0.13136950406608935 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC | 11002 | 0.1235111334588203 | TruSeq Adapter, Index 3 (100% over 50bp) |
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG | 9736 | 0.10929870890338796 | No Hit |
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC | 9734 | 0.10927625641593862 | No Hit |
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC | 9447 | 0.1060543244669583 | No Hit |
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA | 9122 | 0.10240579525644053 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 2190 | 0.0 | 39.79453 | 44 |
TATGCCG | 2280 | 0.0 | 37.916676 | 46 |
GTATGCC | 2365 | 0.0 | 37.44187 | 45 |
CACTTAG | 2440 | 0.0 | 37.007584 | 31 |
GCACACG | 2495 | 0.0 | 36.612206 | 11 |
CTCGTAT | 2490 | 0.0 | 34.2972 | 42 |
ACGTCTG | 2650 | 0.0 | 34.206406 | 15 |
TAGGCAT | 2590 | 0.0 | 33.377262 | 35 |
TTAGGCA | 2725 | 0.0 | 32.879635 | 34 |
CACACGT | 2800 | 0.0 | 32.8739 | 12 |
TCGTATG | 2695 | 0.0 | 32.727463 | 43 |
ATGCCGT | 2685 | 0.0 | 32.45811 | 47 |
AGCACAC | 3080 | 0.0 | 30.22643 | 10 |
TCACTTA | 3030 | 0.0 | 29.801485 | 30 |
CACGTCT | 3095 | 0.0 | 29.401278 | 14 |
ACACGTC | 3295 | 0.0 | 27.829115 | 13 |
GCCGTCT | 3180 | 0.0 | 27.515419 | 49 |
CTAGCAG | 4040 | 0.0 | 26.682425 | 8 |
CATCTCG | 3180 | 0.0 | 26.414803 | 39 |
GGCATCT | 3220 | 0.0 | 26.194921 | 37 |