FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7837944
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG295610.37715247774161187No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC169820.21666396187571638No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG156860.20012901342494918No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT149170.19031776700624553No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC128070.16339744198223413No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG121550.1550789339653358No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG119510.15247621059808542No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC116650.14882729450478338No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG114790.14645422319934923No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA102850.13122063643220722No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC92590.11813046890868319No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT91170.11631876930991086No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC90070.11491534004325625No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA81350.10378997349304868No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA80370.1025396456009382No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG80290.10243757801790877No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC79620.10158276201003734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTC41250.028.597742
CTAGCAG43150.027.1712348
GCTGACT44450.026.8585911
CGTATGC10700.023.87878244
CCGGGGT4400.023.0752241
TAGCAGA51200.022.9674079
GTATGCC11600.022.6294345
ACTCTAG59500.021.826295
TATGCCG11450.021.39735646
GACTCTA62150.021.008294
CTCTAGC59600.020.3800966
GTGCGCA98750.020.0975968
TGCGCAT100800.019.68898869
AGGTGCG103950.019.19263366
ACGTCTG14000.018.74890315
GGTGCGC109000.018.49621867
AGATCTA64450.018.1916813
ATGCCGT13700.018.13831947
CTATCGA64400.018.04276817
GATCTAT65250.018.02204914
Produced by FastQC (version 0.11.5)