FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_044.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_044.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6855643
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG222990.32526489491941163No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC141940.20704111926481586No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG127560.1860656979950677No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT117250.17102699192475454No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC101450.14798028427092833No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC95000.1385719763995879No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG92610.1350857972038509No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG91190.13301451081977286No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG86290.1258671141423204No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA78920.1151168460784787No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC70410.10270371429784195No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC69400.10123047539085685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG33850.025.330578
CTGACTC35350.024.9560222
GCTGACT36300.023.921681
GACTCTA42900.021.0531544
ACTCTAG44800.020.78545
GTGCGCA78300.019.35608768
TAGCAGA45950.019.1171259
TGCGCAT80900.018.8206869
CTCTAGC47050.018.4521436
CGTATGC8000.018.37518544
AGGTGCG84650.018.02761566
GGTGCGC85850.017.93870567
CTATCGA50650.016.99819217
TATGCCG8600.016.68621346
TCTATCG52200.016.29219616
GTATGCC9250.015.8920545
TCTAGCA86250.015.7843997
CGAGCGC30500.015.7210159
GATCTAT55050.015.38504414
CCGGGGT5050.015.2537981
Produced by FastQC (version 0.11.5)