FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15260817
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG533550.3496208623692952No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC419900.27514909588392283No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG347390.2276352570114693No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT314200.20588674905150883No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC248150.1626059731926541No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC243010.15923787042332005No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG228760.14990023142273443No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG225170.14754780166749917No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG212340.1391406502024105No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA207350.13587083836992475No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA183400.1201770521198177No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC176180.11544598169285433No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC175480.11498729065422905No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA166910.10937160179563127No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG161960.10612800087963835No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC156210.10236018163378802No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG155110.10163938143023404No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT153950.10087926485194075No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC153430.10053852293753342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC23950.030.98131644
CTGACTC82600.029.9620482
CTAGCAG82900.029.5945428
GTATGCC25850.028.70415945
TATGCCG25800.028.35271646
GCTGACT92450.027.2666441
ACTCGGC28650.026.01992232
GCACACG31000.025.40206111
TAGCAGA103300.023.8855679
ACTCTAG107350.023.7709335
GACTCTA110200.023.6960834
ACGTCTG33150.023.4379215
CACACGT33450.023.01845212
TCGTATG32700.022.58439643
CTCGTAT32400.022.46928642
ATGCCGT32150.022.4256947
CTCTAGC114150.022.1708896
AGCACAC38050.021.52325210
CTCGGCA35100.021.13897933
CTATCGA124500.019.5375817
Produced by FastQC (version 0.11.5)