FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8001652
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG232660.29076495703637195No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC173020.21623034843304859No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG147380.1841869653916466No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT132870.1660532100121325No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC101150.12641139604671636No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC98830.12351199477307936No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG95020.11875047802628756No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG88780.11095208839374669No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG87420.10925243937126984No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA85470.1068154426111008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12750.026.07774446
CGTATGC12900.026.04631244
CTAGCAG36550.025.375378
CTGACTC37300.024.9627152
GTATGCC13950.024.08583845
GCTGACT41950.022.5338271
CTCGTAT16250.021.75422542
ACGTCTG16250.021.32251415
GCACACG16150.021.02085311
TAGCAGA46550.020.4503339
ATGCCGT16450.020.42486847
GTGCGCA80450.020.36159168
ACTCTAG48850.020.0633935
TCGTATG16600.020.0304743
GACTCTA51300.019.9922264
TGCGCAT82500.019.8132169
AGGTGCG86250.018.95093566
CACACGT18250.018.7939112
GGTGCGC90200.018.31540567
CACGTCT18750.018.2925114
Produced by FastQC (version 0.11.5)