FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7451324
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG285860.38363651882537925No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC173390.23269690057766915No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG162730.21839071821330006No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT144960.19454260746143906No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC126040.1691511468297446No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG118120.15852216330950042No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC115610.15515363444134223No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG109050.1463498299094228No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG104630.14041799819736733No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA92450.1240719099048706No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC86070.11550967318022945No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT83110.11153722479387555No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC80750.10837000243178259No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA77110.10348496455126632No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC75340.1011095477796966No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA75020.10068009390009078No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT74720.10027748088796032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT44050.029.4083631
CTGACTC42400.029.3092482
CTAGCAG45300.027.9669238
TAGCAGA51550.024.57659
ACTCTAG54450.024.429925
GACTCTA57450.023.7027554
CTCTAGC56900.022.7012846
CCGGGGT5050.021.4924221
CGTATGC8200.020.48745244
CTATCGA60800.020.37739217
TCTATCG63850.019.4036116
GTGCGCA97050.018.9704268
GATCTAT65150.018.96296714
AGCAGAT68000.018.68287310
TGCGCAT98850.018.58982369
AGATCTA68300.018.34460313
TATGCCG9550.018.32429146
AGGTGCG103000.017.87431766
GTATGCC9600.017.86427545
ATCTATC69850.017.83731815
Produced by FastQC (version 0.11.5)