FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_040.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_040.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8636082
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG261370.30264881690562917No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC169070.19577164737435332No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG148150.17154769952392762No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT126110.14602686727615602No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC118410.13711078704440277No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG105260.12188397470056445No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC105110.1217102848259199No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG101070.11703223753549352No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG97510.1129099978439297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG39800.024.7976288
GCTGACT41300.024.2446581
CTGACTC42850.023.281242
CGTATGC11450.023.23204644
GTGCGCA90250.022.02929368
TGCGCAT92950.021.31420169
ACTCTAG54100.021.221995
GACTCTA53450.020.759464
AGGTGCG97650.020.25212766
GGTGCGC99300.020.1624767
CGCGGGG4200.020.006081
TATGCCG13150.019.4299446
GTATGCC14050.018.43453645
TAGCAGA54550.018.2851839
CTCTAGC55800.018.1917026
CCGGGGT5750.017.6575411
AAGGTGC118400.016.79185365
GCACACG15950.016.67668211
CTATCGA59050.016.65484617
TCGTATG17000.016.47101643
Produced by FastQC (version 0.11.5)