FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_039.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_039.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8901111
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG339360.3812557780708498No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC220980.24826114403022273No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT186440.20945699924425162No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG180000.20222194735016785No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC143760.16150792861700072No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG137460.15443016045974486No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC135500.15222818814415415No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG134880.1515316458810591No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG127840.14362252082914145No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA116870.13129821659341176No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC104790.1177268770156894No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC99540.11182873688464284No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA98480.11063787430580295No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT94750.10644738617460224No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG94210.10584072033255174No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA92010.10336911875382745No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC90440.10160529399082878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTC49450.029.1641242
CTAGCAG49850.028.9949518
GCTGACT54500.026.467411
ACTCTAG71050.023.204775
CGTATGC11450.022.92621244
TAGCAGA65800.022.4984179
GACTCTA68800.022.3354174
CTCTAGC68300.022.0891726
GTGCGCA120100.021.24652368
TGCGCAT122300.020.7500869
TATGCCG11500.020.6961746
GTATGCC12200.020.3690245
AGGTGCG127800.019.8844766
GGTGCGC130500.019.8752967
TCTATCG72650.019.75151316
CTATCGA75000.019.039317
GATCTAT78650.018.33299814
ATCTATC79800.018.24443215
AGATCTA80900.018.21246113
GCATGAC79450.018.0609129
Produced by FastQC (version 0.11.5)