FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9084631
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG224790.24743987950638832No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC160390.17655092430281427No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT126130.13883888074265208No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG118250.13016489057177996No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG96300.10600320475317049No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC95710.10535375625052906No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG93040.10241472658603305No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA92710.10205147572862343No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG92240.10153411844685822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC13150.028.74575445
TATGCCG13200.028.63671346
CGTATGC14450.026.40185444
CTAGCAG36500.023.9715778
ATGCCGT16950.023.7460847
GCACACG17300.023.66976511
CTGACTC38750.022.7645762
CACACGT19000.021.73602912
ACGTCTG19750.020.55596715
CTCGTAT18250.020.520842
GTGCGCA75450.020.1799568
TGCGCAT76850.019.90330769
AGCACAC21300.019.88199410
CGCGGGG4600.019.7886221
TCGTATG19550.019.51441243
CCGGGGT5250.018.6723421
AGGTGCG83000.018.38627266
CACGTCT22500.018.19932214
TAGCAGA49800.017.7802649
ACTCTAG68950.017.565895
Produced by FastQC (version 0.11.5)