FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6489892
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG246320.3795440663727532No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC166930.25721537430823194No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG145940.22487277138047906No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT122200.18829280980330643No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC109650.16895504578504542No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG102960.1586467078342752No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC102100.15732157022027488No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG98780.15220592268715719No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG98750.15215969695643625No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA94460.14554941746334146No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC81290.12525632167684764No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA80030.12331484098656803No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC79960.12320698094821916No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT76080.11722845310831059No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG72010.11095716230716936No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA71190.10969365900079693No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC67730.10436229139098153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7900.028.35380244
CTGACTC33400.027.5639442
CTAGCAG34200.026.4025178
GCTGACT36000.025.8701061
TATGCCG8700.025.34368146
GTATGCC8750.025.19924745
GTGCGCA81500.022.20264868
ACTCTAG44600.022.1331395
TGCGCAT83650.021.67399669
TAGCAGA43250.021.6062939
GCACACG11150.021.34465611
AGGTGCG86950.020.81099166
GACTCTA46800.020.7935034
ACGTCTG10900.020.54952815
GGTGCGC90500.020.2653767
ATGCCGT11100.020.17911147
CTCGTAT11600.019.61178642
CTCTAGC48100.019.1398876
TCGTATG12000.018.95791443
CTATCGA51150.017.7902817
Produced by FastQC (version 0.11.5)