FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_036.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_036.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5250212
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC65990.12569016260676713TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC14100.038.72384645
CGTATGC14150.037.59760344
TATGCCG14900.036.17490846
GCACACG16100.034.34625611
CTCGTAT16500.032.45522342
TCGTATG16750.031.7618643
ACGTCTG17950.030.80609715
CACACGT17850.030.78290712
CACGTCT19400.028.50330714
ACACGTC20200.027.54798513
CGGAATA19850.027.15344634
TATCTCG20100.025.77045639
AGCACAC21600.025.76246510
GCCGTCT21150.024.16011249
CCAGTCA23550.023.48244726
CAGTCAC22600.023.38544727
TCTCGTA23700.022.89077441
TCCAGTC25850.021.79929525
AGTCACC25150.021.15332228
GAGCACA26850.020.5951569
Produced by FastQC (version 0.11.5)