FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6400386
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC11800.029.06679345
TATGCCG12550.026.77219446
GCACACG14550.025.73734311
CGTATGC13300.025.52543444
GTCACCC13950.023.58378229
ACGTCTG16000.021.87356215
CACACGT17900.020.52953712
ACACGTC18650.019.3286413
CTCGTAT16700.018.8619242
TCGTATG17900.018.77030443
AGCACAC20650.018.30402610
GCTGACT23650.018.209161
CACGTCT19200.017.86340914
GCGGGGT8100.015.9930991
CTAGCAG23450.015.6709798
ACTCTAG28600.015.6664885
CACCCAA23700.015.21056531
GACTCTA27500.015.0202464
CGGGGGG3503.0377123E-1015.0051091
CGTCTGA24550.014.96846616
Produced by FastQC (version 0.11.5)