FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9647596
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC101200.10489659807479501TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22350.036.0171344
GCACACG23900.035.5842811
TATGCCG22350.035.39111346
GTATGCC22600.035.30898345
ACGTCTG28000.030.7487115
CTCGTAT26100.030.70879742
TCGTATG26250.030.39998243
CACACGT28850.029.60029812
AGCACAC31000.027.88592710
CACGTCT31600.027.24540914
TATCTCG32000.025.15558439
GCCGTCT33300.023.6477849
GAGCACA37750.023.5483139
ACACGTC37950.022.50246613
AGTCACC36750.022.38128928
CAGTCAC37700.022.18866327
TCCAGTC40350.021.77232425
CCAGTCA40100.021.47197726
CATTTTA40500.020.73976134
TCTCGTA40450.019.72815141
Produced by FastQC (version 0.11.5)