FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_033.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_033.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6459538
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16750.029.46211811
ACGTCTG18450.026.17775215
GTATGCC11050.024.7059845
CACACGT20200.024.43022312
CGTATGC11350.024.05295844
TATGCCG10700.023.5518646
CACGTCT21750.022.8497814
ACACGTC22450.021.82586113
AGCACAC22650.021.7878310
CAGTCAC24550.019.3893827
GAGCACA25800.018.8560819
AGTCACC25300.018.81430428
TCCAGTC27050.018.50313225
CCAGTCA26600.017.8946726
CTCGTAT14500.017.13799942
CATGGCA28400.016.6364934
TCGTATG14450.016.22856543
ATGGCAT29500.016.13490935
CTAGCAG22750.015.5378028
CATCTCG19500.015.43524239
Produced by FastQC (version 0.11.5)