FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4799020
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC67160.13994523881959234TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13550.040.2948844
GTATGCC14000.038.24969545
TATGCCG13850.037.90543746
GCACACG14800.037.36417811
CCAGGCG14650.036.31332433
CAGGCGA15300.033.85558734
CTCGTAT15600.032.7561542
TCGTATG16050.032.71001443
CACACGT17200.032.55753712
ACGTCTG17500.031.99874315
ACCAGGC17100.031.31488432
AGCACAC18100.030.74528910
AGGCGAT18300.028.11452935
CACGTCT20200.027.72197314
GCCGTCT19950.027.1924849
ACACGTC21750.025.90729713
GAGCACA21800.025.5267859
TCCAGTC23150.025.09725425
AGTCACC21200.025.09413728
CAGTCAC21300.024.97658327
Produced by FastQC (version 0.11.5)