FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8101242
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15550.031.96097246
GCACACG16800.031.66583811
GTATGCC16750.030.50704245
CGTATGC17200.029.50558944
CACACGT20450.026.52725612
ACGTCTG20200.026.5088815
TCGTATG19450.026.09234643
CTCGTAT19550.024.70569442
AGCACAC22150.024.64946710
CACGTCT22100.024.22968714
GAGCACA29250.019.383879
CTAGCAG27100.018.8552468
GCCGTCT27250.018.75133949
AGTCACC27500.018.4542928
ACACGTC28950.018.37591713
CCACTCA27600.018.00677533
TCCAGTC30350.017.75966625
CCAGTCA29200.017.73991826
CAGTCAC29600.017.7364627
TACTCTA29000.017.3814
Produced by FastQC (version 0.11.5)