FastQCFastQC Report
Wed 7 Nov 2018
HFKMCBGX9_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKMCBGX9_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255127454
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138017325.409740027429584No Hit
CTGTCTCTTATACACATCTGAGGCGGAGACGGTGAGGCTATACGCACCTT9544390.37410281999678485No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA4027330.1578556104745983No Hit
GATATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGG3406720.13353012177199872No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC2721750.10668197237605012No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC2715310.10642954952233405No Hit
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACTCCTACCTATA2657320.10415656795603032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG1044250.042.3732538
TATACGC1046700.042.28424539
TACGCAC1052300.041.9931641
ATACGCA1070250.041.33271840
GCTATAC1102700.040.1660137
GGCTATA1146200.038.73110636
ACGCACC1143900.038.5649542
ACGGTGA1164150.038.2204629
GACGGTG1215700.038.01683828
TCGCATA354550.036.85764340
CGGAGAC1269550.036.69996624
CGACGAT136850.036.59930838
ATCGCAT363800.035.93911439
TAGCGTG463550.035.79516215
CGGTGAG1252500.035.56294330
CGGTAAT377400.035.1663934
CGTGGTA478150.034.6518818
CACGCTA400750.034.37470632
GGAGACG1365700.034.13884425
CCGGTAA388050.034.1221633