Basic Statistics
Measure | Value |
---|---|
Filename | HFKMCBGX9_4.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 255127454 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 13801732 | 5.409740027429584 | No Hit |
CTGTCTCTTATACACATCTGAGGCGGAGACGGTGAGGCTATACGCACCTT | 954439 | 0.37410281999678485 | No Hit |
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA | 402733 | 0.1578556104745983 | No Hit |
GATATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGG | 340672 | 0.13353012177199872 | No Hit |
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC | 272175 | 0.10668197237605012 | No Hit |
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC | 271531 | 0.10642954952233405 | No Hit |
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACTCCTACCTATA | 265732 | 0.10415656795603032 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTATACG | 104425 | 0.0 | 42.37325 | 38 |
TATACGC | 104670 | 0.0 | 42.284245 | 39 |
TACGCAC | 105230 | 0.0 | 41.99316 | 41 |
ATACGCA | 107025 | 0.0 | 41.332718 | 40 |
GCTATAC | 110270 | 0.0 | 40.16601 | 37 |
GGCTATA | 114620 | 0.0 | 38.731106 | 36 |
ACGCACC | 114390 | 0.0 | 38.56495 | 42 |
ACGGTGA | 116415 | 0.0 | 38.22046 | 29 |
GACGGTG | 121570 | 0.0 | 38.016838 | 28 |
TCGCATA | 35455 | 0.0 | 36.857643 | 40 |
CGGAGAC | 126955 | 0.0 | 36.699966 | 24 |
CGACGAT | 13685 | 0.0 | 36.599308 | 38 |
ATCGCAT | 36380 | 0.0 | 35.939114 | 39 |
TAGCGTG | 46355 | 0.0 | 35.795162 | 15 |
CGGTGAG | 125250 | 0.0 | 35.562943 | 30 |
CGGTAAT | 37740 | 0.0 | 35.16639 | 34 |
CGTGGTA | 47815 | 0.0 | 34.65188 | 18 |
CACGCTA | 40075 | 0.0 | 34.374706 | 32 |
GGAGACG | 136570 | 0.0 | 34.138844 | 25 |
CCGGTAA | 38805 | 0.0 | 34.12216 | 33 |