FastQCFastQC Report
Wed 7 Nov 2018
HFKMCBGX9_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKMCBGX9_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255127454
Sequences flagged as poor quality0
Sequence length43
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9391350036.8104249572451No Hit
ATTACTCGCCTGTCCCTGTCGGCTCGGGTGCTCTGGTTCTTAG2901837011.374067958989627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGA3750.037.0649871
GCTAGAT1700.037.0649871
ATTTGTC1700.037.0649871
GCAAGTT650.037.0649831
CTGTAGA200.00182688937.0649831
TTAGCGT750.037.0649831
ATTGTCC308.219675E-637.0649831
TTGAACT200.00182688937.0649831
CTAGCTT452.581146E-937.0649831
CTGAAGC852350.037.056291
ATTACTC31272100.037.0550271
CTTCTAG935000.037.0530931
CTCCAGA1992450.037.0501021
AAGTTCC2286600.037.048781
ACTGCTC1243150.037.0470921
CTACGTC1742800.037.0458451
TCCTGAG843550.037.045211
ATCTCTG403.7984137E-837.044722
CCAGATT1600.037.044722
TGTTGCC950.037.044722
Produced by FastQC (version 0.11.5)