FastQCFastQC Report
Wed 7 Nov 2018
HFKMCBGX9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKMCBGX9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255127454
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82811723.245896068872306No Hit
CTGTCTCTTATACACATCTGCCGTCCTCGATCGCATTACTCGCCTGTCCC9493490.3721077387461406No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA4046320.15859994432429841No Hit
GATATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGG3782970.14827765262769407No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC2903670.11381252603257663No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC2732260.10709392333762716No Hit
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACTCCTACCTATA2612870.10241430152005515No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT2560050.10034396376643966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA1070150.042.15186732
TCGATCG1163900.040.1472327
GATCGCA1162200.038.93789729
TCCTCGA1206200.038.83691824
CTCGATC1248300.037.64946726
TACTCGC1204500.037.24809337
GTCCTCG1262600.037.1905423
TAGCGTG500300.037.05026615
TCGCATA359650.037.01602640
CGATCGC1264250.036.96193328
ACTCGCC1231100.036.46280338
CGGTAAT377700.035.92431634
CGTCCTC1333600.035.5000522
GCATTAC1271500.035.48637833
CGTGGTA524050.035.3672218
CTTGAAA1705650.034.97177544
CCGGTAA395300.034.25752333
TCGCCTG1316550.034.2116340
TCGCATT1333250.034.0362531
CCCGGTA400400.033.73243332