FastQCFastQC Report
Fri 19 Aug 2022
HFKLKDMXY_n01_CA2100L85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKLKDMXY_n01_CA2100L85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51223105
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGAGCTATCTCGTAT1756570.3429253263737136TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGAGCTATCGCGTAT651190.12712817780179472TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)