FastQCFastQC Report
Mon 22 Aug 2022
HFKFTDMXY_n02_U1-28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKFTDMXY_n02_U1-28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14516914
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACC363140.2501495841333771Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACC362450.24967427650256796Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAC310500.21388843386411188Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACCT245490.1691061888222249Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC236660.16302362885114566Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCA184680.12721712066352395Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCA184050.12678314413104602Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG181910.12530900162389885Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACCTT154150.10618648012931675Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACCTT152150.1048087768516091Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)