FastQCFastQC Report
Mon 22 Aug 2022
HFKFTDMXY_n02_CA1100L24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKFTDMXY_n02_CA1100L24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55514394
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTA1520060.27381367073916Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTA1497830.269809303871713Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTAA926710.16693148086962814Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTT875470.1577014422601821Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTAAG833770.15018987688130037Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTAAG830470.14959543645563347Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGT680280.12254119174929659Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGT677120.12197197000835494Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)