FastQCFastQC Report
Mon 22 Aug 2022
HFKFTDMXY_n01_U1-28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKFTDMXY_n01_U1-28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14516914
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA362140.24946073249452327TruSeq Adapter, Index 2 (100% over 36bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA361870.24927474255203275TruSeq Adapter, Index 2 (100% over 36bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG307030.21149811867728913TruSeq Adapter, Index 2 (100% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATTGCATCTCGTAT296140.203996524330171TruSeq Adapter, Index 22 (97% over 37bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAA241490.1663507822668096TruSeq Adapter, Index 22 (97% over 37bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC233870.16110173277874348Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC184260.12692780297520534Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC183840.12663848528688673Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC179690.12377975098564337Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAT153830.10596604760488351TruSeq Adapter, Index 22 (97% over 37bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAT153220.10554584810518268TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)