FastQCFastQC Report
Mon 22 Aug 2022
HFKFTDMXY_n01_CA1100L24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKFTDMXY_n01_CA1100L24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55514394
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG1535320.2765625073742136TruSeq Adapter, Index 7 (97% over 36bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG1510000.27200152810818756TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG928100.16718186638225754TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA883140.15908306591620186TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGA841530.1515877125489292TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGA838010.15095364276155118TruSeq Adapter, Index 7 (97% over 36bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT689100.12412996888698813Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT688520.12402549147884062Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)