FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs9_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs9_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10532787
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13750.010.07932311
ACACGTC15950.08.96491613
ACGTCTG15950.08.96491615
CGTCTGA17500.08.67370416
CACGTCT16950.08.56579714
CACACGT16700.08.29884412
AGCACAC17000.08.28179710
GATCGGA16750.07.49068741
CAGTCAC19450.07.35165727
GAGCACA22050.07.08342469
CACATGT22100.06.96782531
AGTCACA20800.06.874506528
TCCAGTC21150.06.864754725
TGGGGGG12600.06.63756285
AGAGCAC24050.06.4943678
CCAGTCA23550.06.258573526
ACATGTC25250.06.18569432
TCACATG23700.06.12614230
GTCACAT23550.06.071750629
AGGGGGG10702.2737368E-105.96499165
Produced by FastQC (version 0.11.2)