FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs8_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs8_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9531705
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA175480.1841013753572944TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT116450.1221712170068209TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG36000.028.8587721
GCACACG44500.023.2344512
ACACGTC44900.023.1254514
CACACGT46300.022.2361413
CACGTCT47600.021.90614115
GATCGGA47450.021.7990952
ACGTCTG47550.021.74412216
CGTCTGA48900.021.32376917
AGCACAC49650.020.73581511
GTCACAG50000.020.59077530
AGTTCCG48550.020.4806535
CAGTCAC50250.020.40077228
GAGCACA51100.020.23352210
GTTCCGT49200.020.12064736
GAACTCC51950.020.029522
AGTCACA52650.019.72152129
CCAGTCA52550.019.38218727
AGAGCAC53850.019.3637499
TCCAGTC52500.019.35874426
CCGTATC46500.019.1132439
Produced by FastQC (version 0.11.2)