FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs7_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs7_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7559310
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG9200.07.1734311
ACACGTC10500.06.9138213
CACACGT10500.06.7043112
ACGTCTG10951.0913936E-116.22789115
CACGTCT12100.06.181401714
GTCACAG14150.05.752262629
CACAGTC13653.6379788E-125.64064631
TGGGGGG7854.2726642E-65.6072035
CGTCTGA11153.8653525E-95.524291516
GAGCACA13301.8262654E-95.1275089
TCCAGTC14254.0381565E-105.094393725
AGTCACA14952.928573E-105.003007428