FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs6_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs6_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6907304
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG7600.019.39346711
ACACGTC9700.015.6484613
CACACGT10600.014.11228412
GATCGGA10350.014.0363991
ACGTCTG10850.013.98986715
CACGTCT11500.013.58171914
CGTATGC11250.013.10183844
CGTCTGA11450.012.8725216
AGCACAC11600.012.70606410
CGGGGGG2402.2901622E-711.920465
GAACTCC12700.011.60562321
GTCACCT15250.010.53053429
GAGCACA15150.010.4548379
TCCAGTC15250.010.3862825
CAGTCAC14650.010.21093527
TCGGAAG15950.010.072173
TCGTATG15750.09.9171743
AGTCACC14750.09.84342428
CCAGTCA15650.09.83961126
ACTCCAG16400.09.65797323
Produced by FastQC (version 0.11.2)