FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs4_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs4_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7984522
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC89680.11231730590760473TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC15050.029.38027829
GCACACG16100.027.46382911
TCACGCC16850.026.50284430
AGTCACG16700.026.47743628
ACACGTC17850.025.26425713
CACGCCA18100.024.79407731
GATCGGA18500.023.43971
CACACGT19500.023.23932612
CGTATGC19250.022.85604544
CACGTCT19350.022.50998114
ACGTCTG20200.021.88948615
TATCTCG20600.021.35806339
AGCACAC21450.021.2293510
CAGTCAC22100.020.50541727
CGTCTGA22000.020.1985216
CTCGTAT22050.019.85404642
GAACTCC22550.019.70587321
CCAGTCA22700.019.47887426
GAGCACA23400.019.4602369
TCGTATG22850.019.35148843
Produced by FastQC (version 0.11.2)