FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs3_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs3_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8100836
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG9950.012.60226311
GTCACAC11300.011.48618229
CGGGGGG2606.458067E-711.0031485
CACGTCT12600.010.12638114
CACACGT12350.09.79698812
ACACGTC13050.09.77719513
GATCGGA13050.09.2764021
ACGTCTG13600.09.22003815
GATCTCG11500.09.18211839
CACACAG14100.08.73722931
TGGGGGG8850.08.7030535
CGTCTGA14450.08.52549616
AGCACAC16750.08.40546910
AGTCACA16950.07.917030328
CAGTCAC16150.07.900506527
CGTATGC15400.07.856868344
GAACTCC17750.07.808033521
GAGCACA17400.07.45932539
TCGTATG17600.07.374742543
CACAGTG17350.07.100572633
Produced by FastQC (version 0.11.2)