FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs2_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs2_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7849242
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG15050.017.403491
CGTATGC16250.017.32850644
GCACACG17650.015.82886211
ACTGACC19950.014.22478732
CACACGT20000.014.18895312
ACACGTC20300.013.76253213
ACGTCTG21000.013.61804415
CACGTCT21500.013.19902714
GATCGGA20750.013.153141
AGCACAC21850.012.786242510
CGTCTGA23150.012.63837716
CTCGTAT23000.011.95602142
CAGTCAC24300.011.94988927
GAACTCC24150.011.75076121
TCCAGTC25200.011.697700525
ATCTCGT23150.011.49829240
GAGCACA24950.011.4622999
CGGGGGG2352.2952972E-611.2380835
CCAGTCA26250.011.14591626
TCGTATG26000.011.08415243
Produced by FastQC (version 0.11.2)