FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs22_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs22_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147090
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2850.017.75309811
GTCACAC3750.014.07897929
ACACGTC3800.013.31482313
CACACGT3950.012.80919612
ACGTCTG4400.011.49916615
GATCGGA4101.8189894E-1211.2739231
CGTCTGA5000.010.55923416
CACGTCT4651.8189894E-1210.40784614
AGCACAC5151.8189894E-129.8245310
CAGTCAC5750.09.56452427
TATCTCG5405.456968E-129.3696941
GAACTCC5651.8189894E-129.34445521
GAGCACA5853.6379788E-129.0249869
CACACTG5955.456968E-128.87330531
CTCGTAT6109.094947E-128.65510944
TCACACT5954.5474735E-118.50358530
AGAGCAC6352.1827873E-118.3143578
AGTCACA6605.0931703E-117.999419728
ATCTCGT6758.185452E-117.82165542
TCCAGTC6054.8876245E-97.635809425
Produced by FastQC (version 0.11.2)