FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs20_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs20_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12042357
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT157820.13105407853296494TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG24450.031.2207333
GCACACG27100.029.06058711
ACACGTC29150.027.0923413
GATCGGA28150.026.9771771
CGTACGT28900.026.48950434
CACACGT30200.025.78617712
CACGTCT32000.025.02315114
ACGTCTG32250.024.6245415
CGTCTGA32550.024.39758516
ACCGTAC34300.022.70395932
GTACGTA34700.022.31544935
GAACTCC35650.022.27596321
AGCACAC35450.022.2155710
GAGCACA37250.021.1421539
TCCAGTC37700.020.48124725
GTCACCG38150.020.18208329
AGAGCAC38850.020.1581848
CAGTCAC38650.020.03482827
CTCGTAT35500.019.89231544
CCAGTCA39500.019.6593126
Produced by FastQC (version 0.11.2)