FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs1_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs1_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8138907
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG3756.693881E-1010.5635985
GCACACG10300.010.03818311
CACACGT12050.08.76291612
ACACGTC13100.08.56432913
ACGTCTG13200.08.16613715
CGTCTGA13250.07.969293616
CGTATGC13200.07.833065544
TGGGGGG10100.07.626365
GTCACCG16600.07.553772429
CAGTCAC14700.07.482507727
CCGATGT15450.07.261753633
TATCTCG14900.07.23451839
TCGGGGG3400.00152861237.12007144
GATCGGA13650.07.094951
CACGTCT15200.07.091645214
AGCACAC16000.07.012044410
TCCAGTC17600.06.87455325
AGTCACC16850.06.788918528
GAGCACA17150.06.67012079
CACCGAT17450.06.303354331
Produced by FastQC (version 0.11.2)