FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs18_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs18_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8341101
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGG13200.025.8316531
ACGTGGC13050.025.79142632
GCACACG13600.025.39525211
AGTCACG14100.023.87064228
TCACGTG14450.023.7493230
GTCACGT14900.023.03205929
CGTGGCC14850.022.51705433
ACACGTC15600.021.8574213
CACACGT16250.021.11849612
GATCGGA16500.020.4102941
ACGTCTG17300.020.21821415
AGCACAC17100.020.19751510
CGTCTGA18450.018.95800216
CACGTCT18600.018.68684414
GTGGCCT19650.017.46451234
GGCCTTA18850.017.2719836
TATCTCG19300.016.98345241
GAACTCC20200.016.77118521
TCCAGTC21300.016.42146725
GAGCACA21650.016.2576039
Produced by FastQC (version 0.11.2)