FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs17_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs17_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6464158
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCT6057.2759576E-128.72683531
AGTCACG6351.8189894E-128.66098328
GTCACGG5456.366463E-108.4766429
TCACGGC6503.6379788E-128.46111430
ACGTCTG8301.8189894E-127.421215
ACGGCTA7801.9444997E-96.768891332
GCACACG7403.3327524E-86.54011211
CGGCTAC6853.7854988E-76.423035633
ACACGTC8358.412826E-96.32293913
CACGTCT9402.6429916E-96.084707314
CAGTCAC12159.749783E-105.43178427
AGCACAC12451.8208084E-95.30085710
CACACGT10003.7308746E-75.27965412
GAGCACA13751.4915713E-105.27965359
TGGGGGG6704.366388E-45.25541975
GATCGGA8401.3967416E-55.2403461
CCAGTCA11851.6643753E-85.197971326
CGTCTGA10002.068915E-65.059707616
Produced by FastQC (version 0.11.2)