FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs16_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs16_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6992136
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC9950.019.01370213
GCACACG9500.018.98810411
CACGTCT12200.015.50707814
GATCGGA11650.015.4930561
CGTCTGA12700.015.4162116
CACACGT12150.015.20877812
ACGTCTG12900.015.00666915
CACTAGC15150.013.21366231
GAACTCC14500.013.04733421
TATCTCG14000.012.88487639
AGCACAC14200.012.85822710
CGTATGC14900.012.54996944
TCACTAG16950.012.45936730
TAGCTTA17500.012.06778734
TCTCGTA15950.011.99930741
TCCAGTC16650.011.89111825
ACTAGCT16900.011.71521532
CGGGGGG1903.700138E-511.5834135
GAGCACA16050.011.5131879
CCAGTCA17900.011.18363126
Produced by FastQC (version 0.11.2)