FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs15_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs15_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4703601
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG4750.011.57824728
GTCACGA5350.010.27975329
GCACACG5900.010.06708111
ACGTCTG7000.09.42790115
CGATCAG6350.09.0073333
ACACGTC6351.8189894E-128.66080313
TAGGGGG2557.197504E-48.6305084
GATCTCG6151.0913936E-118.58493939
CACGATC7103.092282E-117.746011331
CGTCTGA7703.6379788E-127.713737516
CGTATGC7753.6379788E-127.664297644
GAGCACA9750.07.44562539
AGGGGGG4551.2540719E-57.25531675
AGCACAC7904.7293724E-117.23999210
CACACGT7601.4551915E-107.23632812
CAGTCAC8006.184564E-117.14956827
CACGTCT7751.6916601E-96.812419414
ATCTCGT8157.075869E-106.748130340
AGAGCAC10401.8189894E-126.5572268
GAACTCC9651.1277734E-106.38296621
Produced by FastQC (version 0.11.2)