FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs14_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs14_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5061225
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG5150.014.52308211
ACTTAGG6700.012.14849832
CTTAGGC6300.011.52309233
CACACGT6850.011.23995512
CATCTCG6850.010.597671539
ACACGTC7250.010.31639613
ACGTCTG7400.010.1072815
GATCGGA7400.09.816311
CACGTCT7650.09.77697714
CGTCTGA7500.09.67939916
CGTATGC7950.09.408544
CACTTAG8500.09.31706831
AGCACAC9700.09.07142110
TGGGGGG4703.439709E-98.8972845
CAGTCAC9150.08.89562227
GAACTCC8800.08.49947121
GAGCACA11300.07.78697259
TAGGCAT10200.07.764069635
TCCAGTC10650.07.64256625
TTAGGCA10800.07.5364234
Produced by FastQC (version 0.11.2)