FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs12_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs12_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589433
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT109510.23861335376287224TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG17750.032.2234528
GCACACG18200.031.66846511
CACACGT18600.030.98742112
ACGTCTG19100.030.40658815
CACGTGA18850.030.22633631
ACACGTC19000.030.1034913
TCACGTG18950.030.06682830
GTCACGT19050.030.02447729
AGCACAC19350.029.5589810
CACGTCT19750.029.07170314
CGATCTC18150.028.84710140
GATCGGA19600.028.6376111
GAGCACA20250.028.4622089
CAGTCAC20200.028.2059527
CGTCTGA20650.028.0177216
GATCTCG18950.027.6292841
CCAGTCA21650.026.62169626
CTCGTAT20300.026.33399844
TCCAGTC21850.025.97530425
GAACTCC22200.025.9621521
Produced by FastQC (version 0.11.2)