FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs11_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs11_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6287669
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG5800.019.34356932
CGTCCGC6250.016.894933
GTCCGCA8100.014.39401134
GCACACG8250.013.86554311
CCGCACA8050.013.6634936
AGTCACG8850.012.92571428
ACACGTC9650.012.76580613
CACGTCC9150.012.50191831
TCCGCAC9250.012.12874835
GTCACGT9400.011.93539429
CACACGT9800.011.89699512
CGTCTGA10400.011.42215216
CACGTCT10600.010.99910914
TCCAGTC11850.010.58142225
TCACGTC10700.010.48529930
ACGTCTG11450.010.37470615
CATCTCG10850.010.34034141
GATCGGA11050.010.159811
CAGTCAC12400.09.93475927
GCACATC11700.09.40102338
Produced by FastQC (version 0.11.2)