FastQCFastQC Report
Tue 31 Jan 2017
HFKCHAFXX_n01_vs10_170124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKCHAFXX_n01_vs10_170124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7439077
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT182790.24571596718248784TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG35900.030.0871635
GTCCCGA35450.029.04181736
CTCGTAT35200.028.31140344
ACCCGTC37950.028.28818732
CCCGTCC38400.028.12855133
CCGTCCC38850.027.85917334
GCACACG40250.027.65532711
TCACCCG39000.027.5829930
CCCGATC32900.027.48147638
CACCCGT39550.027.19940831
GTCACCC40500.026.5070829
GATCGGA42050.026.3826581
ACACGTC42300.026.31505613
ACGTCTG42600.026.1813815
CACACGT43050.026.11210812
CCGATCT34500.025.8243939
CACGTCT43550.025.55974414
TCCCGAT38000.025.24044637
CGATCTC35600.025.08874740
CGTCTGA44850.024.86793116
Produced by FastQC (version 0.11.2)