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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.9

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2024-06-22, 19:11 based on data in: /vast/gencore/GENEFLOW/work/a3/0f910484095dfd0db681b0ef7086f1/2


        General Statistics

        Showing 258/258 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HFK3YDRX5_l02_n01_AtbZIP28_An1AmpPoolHmB-B_al1
        34.0%
        40%
        3.4
        HFK3YDRX5_l02_n01_AtbZIP28_An1AmpPoolHmB-B_al2
        33.7%
        41%
        3.3
        HFK3YDRX5_l02_n01_AtbZIP28_C24AmpPoolHmB-B_al1
        33.8%
        39%
        4.2
        HFK3YDRX5_l02_n01_AtbZIP28_C24AmpPoolHmB-B_al2
        34.4%
        40%
        4.3
        HFK3YDRX5_l02_n01_AtbZIP28_ColAmpPoolHmB-B_al1
        30.8%
        39%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP28_ColAmpPoolHmB-B_al2
        30.6%
        41%
        3.3
        HFK3YDRX5_l02_n01_AtbZIP28_CviAmpPoolHmB-B_al1
        36.2%
        39%
        3.9
        HFK3YDRX5_l02_n01_AtbZIP28_CviAmpPoolHmB-B_al2
        35.9%
        40%
        3.8
        HFK3YDRX5_l02_n01_AtbZIP28_EriAmpPoolHmB-B_al1
        26.9%
        39%
        3.0
        HFK3YDRX5_l02_n01_AtbZIP28_EriAmpPoolHmB-B_al2
        26.7%
        40%
        2.7
        HFK3YDRX5_l02_n01_AtbZIP28_KyoAmpPoolHmB-B_al1
        31.0%
        39%
        2.7
        HFK3YDRX5_l02_n01_AtbZIP28_KyoAmpPoolHmB-B_al2
        31.3%
        41%
        2.5
        HFK3YDRX5_l02_n01_AtbZIP28_LerAmpPoolHmB-B_al1
        30.9%
        39%
        2.8
        HFK3YDRX5_l02_n01_AtbZIP28_LerAmpPoolHmB-B_al2
        30.8%
        41%
        2.7
        HFK3YDRX5_l02_n01_AtbZIP28_ShaAmpPoolHmB-B_al1
        34.0%
        39%
        4.3
        HFK3YDRX5_l02_n01_AtbZIP28_ShaAmpPoolHmB-B_al2
        33.7%
        40%
        4.1
        HFK3YDRX5_l02_n01_AtbZIP34_An1AmpPoolHmB-B_al1
        42.3%
        40%
        3.2
        HFK3YDRX5_l02_n01_AtbZIP34_An1AmpPoolHmB-B_al2
        37.3%
        41%
        2.8
        HFK3YDRX5_l02_n01_AtbZIP34_C24AmpPoolHmB-B_al1
        41.6%
        40%
        3.6
        HFK3YDRX5_l02_n01_AtbZIP34_C24AmpPoolHmB-B_al2
        37.0%
        40%
        3.3
        HFK3YDRX5_l02_n01_AtbZIP34_ColAmpPoolHmB-B_al1
        41.2%
        40%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP34_ColAmpPoolHmB-B_al2
        35.9%
        40%
        3.0
        HFK3YDRX5_l02_n01_AtbZIP34_CviAmpPoolHmB-B_al1
        43.2%
        40%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP34_CviAmpPoolHmB-B_al2
        38.5%
        40%
        3.1
        HFK3YDRX5_l02_n01_AtbZIP34_EriAmpPoolHmB-B_al1
        37.6%
        39%
        2.9
        HFK3YDRX5_l02_n01_AtbZIP34_EriAmpPoolHmB-B_al2
        32.1%
        40%
        2.5
        HFK3YDRX5_l02_n01_AtbZIP34_KyoAmpPoolHmB-B_al1
        40.5%
        40%
        2.6
        HFK3YDRX5_l02_n01_AtbZIP34_KyoAmpPoolHmB-B_al2
        35.1%
        41%
        2.2
        HFK3YDRX5_l02_n01_AtbZIP34_LerAmpPoolHmB-B_al1
        40.5%
        40%
        2.7
        HFK3YDRX5_l02_n01_AtbZIP34_LerAmpPoolHmB-B_al2
        35.8%
        41%
        2.4
        HFK3YDRX5_l02_n01_AtbZIP34_ShaAmpPoolHmB-B_al1
        42.0%
        40%
        3.8
        HFK3YDRX5_l02_n01_AtbZIP34_ShaAmpPoolHmB-B_al2
        37.5%
        40%
        3.4
        HFK3YDRX5_l02_n01_AtbZIP51_An1AmpPoolHmB-B_al1
        31.1%
        42%
        4.1
        HFK3YDRX5_l02_n01_AtbZIP51_An1AmpPoolHmB-B_al2
        37.5%
        41%
        3.2
        HFK3YDRX5_l02_n01_AtbZIP51_C24AmpPoolHmB-B_al1
        30.5%
        42%
        3.7
        HFK3YDRX5_l02_n01_AtbZIP51_C24AmpPoolHmB-B_al2
        37.0%
        40%
        3.8
        HFK3YDRX5_l02_n01_AtbZIP51_ColAmpPoolHmB-B_al1
        30.4%
        42%
        3.4
        HFK3YDRX5_l02_n01_AtbZIP51_ColAmpPoolHmB-B_al2
        35.0%
        40%
        3.2
        HFK3YDRX5_l02_n01_AtbZIP51_CviAmpPoolHmB-B_al1
        30.4%
        42%
        3.4
        HFK3YDRX5_l02_n01_AtbZIP51_CviAmpPoolHmB-B_al2
        39.2%
        40%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP51_EriAmpPoolHmB-B_al1
        25.4%
        41%
        3.3
        HFK3YDRX5_l02_n01_AtbZIP51_EriAmpPoolHmB-B_al2
        31.3%
        40%
        2.8
        HFK3YDRX5_l02_n01_AtbZIP51_KyoAmpPoolHmB-B_al1
        30.2%
        42%
        2.5
        HFK3YDRX5_l02_n01_AtbZIP51_KyoAmpPoolHmB-B_al2
        34.8%
        40%
        2.4
        HFK3YDRX5_l02_n01_AtbZIP51_LerAmpPoolHmB-B_al1
        31.2%
        42%
        3.2
        HFK3YDRX5_l02_n01_AtbZIP51_LerAmpPoolHmB-B_al2
        34.8%
        41%
        2.6
        HFK3YDRX5_l02_n01_AtbZIP51_ShaAmpPoolHmB-B_al1
        26.4%
        41%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP51_ShaAmpPoolHmB-B_al2
        37.1%
        40%
        3.8
        HFK3YDRX5_l02_n01_AtbZIP52_An1AmpPoolHmB-B_al1
        31.9%
        42%
        4.6
        HFK3YDRX5_l02_n01_AtbZIP52_An1AmpPoolHmB-B_al2
        31.4%
        43%
        3.6
        HFK3YDRX5_l02_n01_AtbZIP52_C24AmpPoolHmB-B_al1
        31.9%
        42%
        3.6
        HFK3YDRX5_l02_n01_AtbZIP52_C24AmpPoolHmB-B_al2
        31.1%
        42%
        3.0
        HFK3YDRX5_l02_n01_AtbZIP52_ColAmpPoolHmB-B_al1
        30.5%
        42%
        3.2
        HFK3YDRX5_l02_n01_AtbZIP52_ColAmpPoolHmB-B_al2
        29.8%
        43%
        2.6
        HFK3YDRX5_l02_n01_AtbZIP52_CviAmpPoolHmB-B_al1
        31.8%
        42%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP52_CviAmpPoolHmB-B_al2
        30.9%
        43%
        2.8
        HFK3YDRX5_l02_n01_AtbZIP52_EriAmpPoolHmB-B_al1
        26.0%
        41%
        3.2
        HFK3YDRX5_l02_n01_AtbZIP52_EriAmpPoolHmB-B_al2
        25.1%
        42%
        2.5
        HFK3YDRX5_l02_n01_AtbZIP52_KyoAmpPoolHmB-B_al1
        31.2%
        42%
        2.5
        HFK3YDRX5_l02_n01_AtbZIP52_KyoAmpPoolHmB-B_al2
        30.5%
        43%
        1.9
        HFK3YDRX5_l02_n01_AtbZIP52_LerAmpPoolHmB-B_al1
        31.9%
        42%
        3.0
        HFK3YDRX5_l02_n01_AtbZIP52_LerAmpPoolHmB-B_al2
        31.2%
        43%
        2.4
        HFK3YDRX5_l02_n01_AtbZIP52_ShaAmpPoolHmB-B_al1
        27.6%
        41%
        3.4
        HFK3YDRX5_l02_n01_AtbZIP52_ShaAmpPoolHmB-B_al2
        27.1%
        42%
        2.8
        HFK3YDRX5_l02_n01_AtbZIP54_An1AmpPoolHmB-B_al1
        29.2%
        41%
        3.1
        HFK3YDRX5_l02_n01_AtbZIP54_An1AmpPoolHmB-B_al2
        27.9%
        41%
        2.7
        HFK3YDRX5_l02_n01_AtbZIP54_C24AmpPoolHmB-B_al1
        30.1%
        40%
        4.2
        HFK3YDRX5_l02_n01_AtbZIP54_C24AmpPoolHmB-B_al2
        29.1%
        41%
        3.8
        HFK3YDRX5_l02_n01_AtbZIP54_ColAmpPoolHmB-B_al1
        28.2%
        40%
        4.2
        HFK3YDRX5_l02_n01_AtbZIP54_ColAmpPoolHmB-B_al2
        26.8%
        41%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP54_CviAmpPoolHmB-B_al1
        30.9%
        40%
        4.1
        HFK3YDRX5_l02_n01_AtbZIP54_CviAmpPoolHmB-B_al2
        29.7%
        41%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP54_EriAmpPoolHmB-B_al1
        23.3%
        40%
        3.4
        HFK3YDRX5_l02_n01_AtbZIP54_EriAmpPoolHmB-B_al2
        22.6%
        40%
        2.9
        HFK3YDRX5_l02_n01_AtbZIP54_KyoAmpPoolHmB-B_al1
        27.8%
        40%
        2.6
        HFK3YDRX5_l02_n01_AtbZIP54_KyoAmpPoolHmB-B_al2
        26.8%
        41%
        2.2
        HFK3YDRX5_l02_n01_AtbZIP54_LerAmpPoolHmB-B_al1
        27.4%
        41%
        2.7
        HFK3YDRX5_l02_n01_AtbZIP54_LerAmpPoolHmB-B_al2
        26.5%
        41%
        2.4
        HFK3YDRX5_l02_n01_AtbZIP54_ShaAmpPoolHmB-B_al1
        28.3%
        40%
        4.4
        HFK3YDRX5_l02_n01_AtbZIP54_ShaAmpPoolHmB-B_al2
        27.5%
        41%
        3.8
        HFK3YDRX5_l02_n01_AtbZIP62_An1AmpPoolHmB-B_al1
        31.6%
        40%
        2.9
        HFK3YDRX5_l02_n01_AtbZIP62_An1AmpPoolHmB-B_al2
        33.7%
        41%
        2.5
        HFK3YDRX5_l02_n01_AtbZIP62_C24AmpPoolHmB-B_al1
        31.6%
        39%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP62_C24AmpPoolHmB-B_al2
        33.0%
        40%
        3.0
        HFK3YDRX5_l02_n01_AtbZIP62_ColAmpPoolHmB-B_al1
        29.6%
        40%
        3.0
        HFK3YDRX5_l02_n01_AtbZIP62_ColAmpPoolHmB-B_al2
        32.1%
        41%
        2.7
        HFK3YDRX5_l02_n01_AtbZIP62_CviAmpPoolHmB-B_al1
        33.2%
        40%
        3.2
        HFK3YDRX5_l02_n01_AtbZIP62_CviAmpPoolHmB-B_al2
        34.1%
        41%
        2.7
        HFK3YDRX5_l02_n01_AtbZIP62_EriAmpPoolHmB-B_al1
        25.6%
        39%
        2.5
        HFK3YDRX5_l02_n01_AtbZIP62_EriAmpPoolHmB-B_al2
        28.0%
        40%
        2.2
        HFK3YDRX5_l02_n01_AtbZIP62_KyoAmpPoolHmB-B_al1
        29.6%
        40%
        2.3
        HFK3YDRX5_l02_n01_AtbZIP62_KyoAmpPoolHmB-B_al2
        32.0%
        41%
        2.1
        HFK3YDRX5_l02_n01_AtbZIP62_LerAmpPoolHmB-B_al1
        29.4%
        40%
        2.3
        HFK3YDRX5_l02_n01_AtbZIP62_LerAmpPoolHmB-B_al2
        32.1%
        41%
        2.2
        HFK3YDRX5_l02_n01_AtbZIP62_ShaAmpPoolHmB-B_al1
        30.8%
        39%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP62_ShaAmpPoolHmB-B_al2
        32.2%
        40%
        2.9
        HFK3YDRX5_l02_n01_AtbZIP72_An1AmpPoolHmB-B_al1
        26.9%
        40%
        2.9
        HFK3YDRX5_l02_n01_AtbZIP72_An1AmpPoolHmB-B_al2
        28.9%
        40%
        4.0
        HFK3YDRX5_l02_n01_AtbZIP72_C24AmpPoolHmB-B_al1
        28.2%
        39%
        4.5
        HFK3YDRX5_l02_n01_AtbZIP72_C24AmpPoolHmB-B_al2
        29.5%
        39%
        6.0
        HFK3YDRX5_l02_n01_AtbZIP72_ColAmpPoolHmB-B_al1
        26.0%
        39%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP72_ColAmpPoolHmB-B_al2
        27.4%
        39%
        4.7
        HFK3YDRX5_l02_n01_AtbZIP72_CviAmpPoolHmB-B_al1
        28.6%
        39%
        3.4
        HFK3YDRX5_l02_n01_AtbZIP72_CviAmpPoolHmB-B_al2
        30.3%
        39%
        4.5
        HFK3YDRX5_l02_n01_AtbZIP72_EriAmpPoolHmB-B_al1
        21.6%
        38%
        2.9
        HFK3YDRX5_l02_n01_AtbZIP72_EriAmpPoolHmB-B_al2
        23.1%
        39%
        3.9
        HFK3YDRX5_l02_n01_AtbZIP72_KyoAmpPoolHmB-B_al1
        25.7%
        39%
        2.5
        HFK3YDRX5_l02_n01_AtbZIP72_KyoAmpPoolHmB-B_al2
        27.5%
        39%
        3.5
        HFK3YDRX5_l02_n01_AtbZIP72_LerAmpPoolHmB-B_al1
        26.3%
        39%
        2.7
        HFK3YDRX5_l02_n01_AtbZIP72_LerAmpPoolHmB-B_al2
        28.0%
        39%
        3.7
        HFK3YDRX5_l02_n01_AtbZIP72_ShaAmpPoolHmB-B_al1
        26.1%
        39%
        4.0
        HFK3YDRX5_l02_n01_AtbZIP72_ShaAmpPoolHmB-B_al2
        27.4%
        39%
        5.3
        HFK3YDRX5_l02_n01_AtbZIP77_An1AmpPoolHmB-B_al1
        31.1%
        41%
        2.9
        HFK3YDRX5_l02_n01_AtbZIP77_An1AmpPoolHmB-B_al2
        31.2%
        42%
        3.1
        HFK3YDRX5_l02_n01_AtbZIP77_C24AmpPoolHmB-B_al1
        30.8%
        40%
        3.7
        HFK3YDRX5_l02_n01_AtbZIP77_C24AmpPoolHmB-B_al2
        31.2%
        41%
        4.0
        HFK3YDRX5_l02_n01_AtbZIP77_ColAmpPoolHmB-B_al1
        28.6%
        40%
        3.1
        HFK3YDRX5_l02_n01_AtbZIP77_ColAmpPoolHmB-B_al2
        28.8%
        41%
        3.1
        HFK3YDRX5_l02_n01_AtbZIP77_CviAmpPoolHmB-B_al1
        32.0%
        40%
        3.2
        HFK3YDRX5_l02_n01_AtbZIP77_CviAmpPoolHmB-B_al2
        32.1%
        41%
        3.3
        HFK3YDRX5_l02_n01_AtbZIP77_EriAmpPoolHmB-B_al1
        23.9%
        40%
        2.5
        HFK3YDRX5_l02_n01_AtbZIP77_EriAmpPoolHmB-B_al2
        24.4%
        41%
        2.5
        HFK3YDRX5_l02_n01_AtbZIP77_KyoAmpPoolHmB-B_al1
        28.9%
        40%
        2.3
        HFK3YDRX5_l02_n01_AtbZIP77_KyoAmpPoolHmB-B_al2
        29.4%
        41%
        2.4
        HFK3YDRX5_l02_n01_AtbZIP77_LerAmpPoolHmB-B_al1
        28.7%
        41%
        2.4
        HFK3YDRX5_l02_n01_AtbZIP77_LerAmpPoolHmB-B_al2
        28.8%
        41%
        2.5
        HFK3YDRX5_l02_n01_AtbZIP77_ShaAmpPoolHmB-B_al1
        29.7%
        40%
        3.4
        HFK3YDRX5_l02_n01_AtbZIP77_ShaAmpPoolHmB-B_al2
        29.9%
        41%
        3.6
        HFK3YDRX5_l02_n01_undetermined
        73.6%
        43%
        51.3
        HFK3YDRX5_l02_n02_AtbZIP28_An1AmpPoolHmB-B_al1
        34.2%
        40%
        3.4
        HFK3YDRX5_l02_n02_AtbZIP28_An1AmpPoolHmB-B_al2
        34.2%
        41%
        3.3
        HFK3YDRX5_l02_n02_AtbZIP28_C24AmpPoolHmB-B_al1
        34.0%
        39%
        4.2
        HFK3YDRX5_l02_n02_AtbZIP28_C24AmpPoolHmB-B_al2
        34.7%
        40%
        4.3
        HFK3YDRX5_l02_n02_AtbZIP28_ColAmpPoolHmB-B_al1
        30.8%
        39%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP28_ColAmpPoolHmB-B_al2
        30.7%
        40%
        3.3
        HFK3YDRX5_l02_n02_AtbZIP28_CviAmpPoolHmB-B_al1
        36.4%
        39%
        3.9
        HFK3YDRX5_l02_n02_AtbZIP28_CviAmpPoolHmB-B_al2
        36.1%
        40%
        3.8
        HFK3YDRX5_l02_n02_AtbZIP28_EriAmpPoolHmB-B_al1
        26.9%
        39%
        3.0
        HFK3YDRX5_l02_n02_AtbZIP28_EriAmpPoolHmB-B_al2
        26.5%
        40%
        2.7
        HFK3YDRX5_l02_n02_AtbZIP28_KyoAmpPoolHmB-B_al1
        30.8%
        39%
        2.7
        HFK3YDRX5_l02_n02_AtbZIP28_KyoAmpPoolHmB-B_al2
        31.0%
        41%
        2.5
        HFK3YDRX5_l02_n02_AtbZIP28_LerAmpPoolHmB-B_al1
        30.8%
        39%
        2.8
        HFK3YDRX5_l02_n02_AtbZIP28_LerAmpPoolHmB-B_al2
        30.7%
        41%
        2.7
        HFK3YDRX5_l02_n02_AtbZIP28_ShaAmpPoolHmB-B_al1
        33.6%
        39%
        4.3
        HFK3YDRX5_l02_n02_AtbZIP28_ShaAmpPoolHmB-B_al2
        33.4%
        40%
        4.1
        HFK3YDRX5_l02_n02_AtbZIP34_An1AmpPoolHmB-B_al1
        41.2%
        40%
        3.2
        HFK3YDRX5_l02_n02_AtbZIP34_An1AmpPoolHmB-B_al2
        36.4%
        41%
        2.8
        HFK3YDRX5_l02_n02_AtbZIP34_C24AmpPoolHmB-B_al1
        40.8%
        40%
        3.6
        HFK3YDRX5_l02_n02_AtbZIP34_C24AmpPoolHmB-B_al2
        36.5%
        40%
        3.3
        HFK3YDRX5_l02_n02_AtbZIP34_ColAmpPoolHmB-B_al1
        40.0%
        40%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP34_ColAmpPoolHmB-B_al2
        35.0%
        40%
        3.0
        HFK3YDRX5_l02_n02_AtbZIP34_CviAmpPoolHmB-B_al1
        42.2%
        40%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP34_CviAmpPoolHmB-B_al2
        37.6%
        40%
        3.1
        HFK3YDRX5_l02_n02_AtbZIP34_EriAmpPoolHmB-B_al1
        36.7%
        39%
        2.9
        HFK3YDRX5_l02_n02_AtbZIP34_EriAmpPoolHmB-B_al2
        31.3%
        40%
        2.5
        HFK3YDRX5_l02_n02_AtbZIP34_KyoAmpPoolHmB-B_al1
        39.5%
        40%
        2.6
        HFK3YDRX5_l02_n02_AtbZIP34_KyoAmpPoolHmB-B_al2
        34.1%
        41%
        2.2
        HFK3YDRX5_l02_n02_AtbZIP34_LerAmpPoolHmB-B_al1
        39.4%
        40%
        2.7
        HFK3YDRX5_l02_n02_AtbZIP34_LerAmpPoolHmB-B_al2
        34.7%
        41%
        2.4
        HFK3YDRX5_l02_n02_AtbZIP34_ShaAmpPoolHmB-B_al1
        41.0%
        40%
        3.8
        HFK3YDRX5_l02_n02_AtbZIP34_ShaAmpPoolHmB-B_al2
        36.4%
        40%
        3.4
        HFK3YDRX5_l02_n02_AtbZIP51_An1AmpPoolHmB-B_al1
        29.9%
        42%
        4.1
        HFK3YDRX5_l02_n02_AtbZIP51_An1AmpPoolHmB-B_al2
        36.5%
        41%
        3.2
        HFK3YDRX5_l02_n02_AtbZIP51_C24AmpPoolHmB-B_al1
        29.9%
        42%
        3.7
        HFK3YDRX5_l02_n02_AtbZIP51_C24AmpPoolHmB-B_al2
        36.9%
        40%
        3.8
        HFK3YDRX5_l02_n02_AtbZIP51_ColAmpPoolHmB-B_al1
        29.1%
        42%
        3.4
        HFK3YDRX5_l02_n02_AtbZIP51_ColAmpPoolHmB-B_al2
        34.4%
        40%
        3.2
        HFK3YDRX5_l02_n02_AtbZIP51_CviAmpPoolHmB-B_al1
        29.5%
        42%
        3.4
        HFK3YDRX5_l02_n02_AtbZIP51_CviAmpPoolHmB-B_al2
        38.4%
        40%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP51_EriAmpPoolHmB-B_al1
        24.3%
        41%
        3.3
        HFK3YDRX5_l02_n02_AtbZIP51_EriAmpPoolHmB-B_al2
        30.7%
        40%
        2.8
        HFK3YDRX5_l02_n02_AtbZIP51_KyoAmpPoolHmB-B_al1
        28.9%
        42%
        2.5
        HFK3YDRX5_l02_n02_AtbZIP51_KyoAmpPoolHmB-B_al2
        33.9%
        40%
        2.4
        HFK3YDRX5_l02_n02_AtbZIP51_LerAmpPoolHmB-B_al1
        29.9%
        42%
        3.2
        HFK3YDRX5_l02_n02_AtbZIP51_LerAmpPoolHmB-B_al2
        34.0%
        41%
        2.6
        HFK3YDRX5_l02_n02_AtbZIP51_ShaAmpPoolHmB-B_al1
        25.2%
        41%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP51_ShaAmpPoolHmB-B_al2
        36.4%
        40%
        3.8
        HFK3YDRX5_l02_n02_AtbZIP52_An1AmpPoolHmB-B_al1
        31.0%
        42%
        4.6
        HFK3YDRX5_l02_n02_AtbZIP52_An1AmpPoolHmB-B_al2
        30.4%
        42%
        3.6
        HFK3YDRX5_l02_n02_AtbZIP52_C24AmpPoolHmB-B_al1
        31.2%
        42%
        3.6
        HFK3YDRX5_l02_n02_AtbZIP52_C24AmpPoolHmB-B_al2
        30.7%
        42%
        3.0
        HFK3YDRX5_l02_n02_AtbZIP52_ColAmpPoolHmB-B_al1
        29.3%
        42%
        3.2
        HFK3YDRX5_l02_n02_AtbZIP52_ColAmpPoolHmB-B_al2
        28.7%
        43%
        2.6
        HFK3YDRX5_l02_n02_AtbZIP52_CviAmpPoolHmB-B_al1
        30.7%
        42%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP52_CviAmpPoolHmB-B_al2
        30.2%
        42%
        2.8
        HFK3YDRX5_l02_n02_AtbZIP52_EriAmpPoolHmB-B_al1
        24.8%
        41%
        3.2
        HFK3YDRX5_l02_n02_AtbZIP52_EriAmpPoolHmB-B_al2
        24.4%
        42%
        2.5
        HFK3YDRX5_l02_n02_AtbZIP52_KyoAmpPoolHmB-B_al1
        30.0%
        42%
        2.5
        HFK3YDRX5_l02_n02_AtbZIP52_KyoAmpPoolHmB-B_al2
        29.3%
        43%
        1.9
        HFK3YDRX5_l02_n02_AtbZIP52_LerAmpPoolHmB-B_al1
        30.7%
        42%
        3.0
        HFK3YDRX5_l02_n02_AtbZIP52_LerAmpPoolHmB-B_al2
        30.0%
        43%
        2.4
        HFK3YDRX5_l02_n02_AtbZIP52_ShaAmpPoolHmB-B_al1
        26.6%
        41%
        3.4
        HFK3YDRX5_l02_n02_AtbZIP52_ShaAmpPoolHmB-B_al2
        26.3%
        42%
        2.8
        HFK3YDRX5_l02_n02_AtbZIP54_An1AmpPoolHmB-B_al1
        28.9%
        41%
        3.1
        HFK3YDRX5_l02_n02_AtbZIP54_An1AmpPoolHmB-B_al2
        27.6%
        41%
        2.7
        HFK3YDRX5_l02_n02_AtbZIP54_C24AmpPoolHmB-B_al1
        30.0%
        40%
        4.2
        HFK3YDRX5_l02_n02_AtbZIP54_C24AmpPoolHmB-B_al2
        28.8%
        41%
        3.8
        HFK3YDRX5_l02_n02_AtbZIP54_ColAmpPoolHmB-B_al1
        27.5%
        40%
        4.2
        HFK3YDRX5_l02_n02_AtbZIP54_ColAmpPoolHmB-B_al2
        26.1%
        41%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP54_CviAmpPoolHmB-B_al1
        30.6%
        40%
        4.1
        HFK3YDRX5_l02_n02_AtbZIP54_CviAmpPoolHmB-B_al2
        29.4%
        41%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP54_EriAmpPoolHmB-B_al1
        22.9%
        40%
        3.4
        HFK3YDRX5_l02_n02_AtbZIP54_EriAmpPoolHmB-B_al2
        22.1%
        40%
        2.9
        HFK3YDRX5_l02_n02_AtbZIP54_KyoAmpPoolHmB-B_al1
        27.0%
        41%
        2.6
        HFK3YDRX5_l02_n02_AtbZIP54_KyoAmpPoolHmB-B_al2
        26.2%
        41%
        2.2
        HFK3YDRX5_l02_n02_AtbZIP54_LerAmpPoolHmB-B_al1
        26.9%
        41%
        2.7
        HFK3YDRX5_l02_n02_AtbZIP54_LerAmpPoolHmB-B_al2
        25.8%
        41%
        2.4
        HFK3YDRX5_l02_n02_AtbZIP54_ShaAmpPoolHmB-B_al1
        27.6%
        40%
        4.4
        HFK3YDRX5_l02_n02_AtbZIP54_ShaAmpPoolHmB-B_al2
        26.8%
        40%
        3.8
        HFK3YDRX5_l02_n02_AtbZIP62_An1AmpPoolHmB-B_al1
        31.5%
        40%
        2.9
        HFK3YDRX5_l02_n02_AtbZIP62_An1AmpPoolHmB-B_al2
        33.0%
        41%
        2.5
        HFK3YDRX5_l02_n02_AtbZIP62_C24AmpPoolHmB-B_al1
        31.6%
        40%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP62_C24AmpPoolHmB-B_al2
        32.7%
        40%
        3.0
        HFK3YDRX5_l02_n02_AtbZIP62_ColAmpPoolHmB-B_al1
        29.2%
        40%
        3.0
        HFK3YDRX5_l02_n02_AtbZIP62_ColAmpPoolHmB-B_al2
        31.4%
        41%
        2.7
        HFK3YDRX5_l02_n02_AtbZIP62_CviAmpPoolHmB-B_al1
        33.0%
        40%
        3.2
        HFK3YDRX5_l02_n02_AtbZIP62_CviAmpPoolHmB-B_al2
        33.4%
        41%
        2.7
        HFK3YDRX5_l02_n02_AtbZIP62_EriAmpPoolHmB-B_al1
        25.3%
        39%
        2.5
        HFK3YDRX5_l02_n02_AtbZIP62_EriAmpPoolHmB-B_al2
        27.4%
        40%
        2.2
        HFK3YDRX5_l02_n02_AtbZIP62_KyoAmpPoolHmB-B_al1
        29.2%
        40%
        2.3
        HFK3YDRX5_l02_n02_AtbZIP62_KyoAmpPoolHmB-B_al2
        31.4%
        41%
        2.1
        HFK3YDRX5_l02_n02_AtbZIP62_LerAmpPoolHmB-B_al1
        29.0%
        40%
        2.3
        HFK3YDRX5_l02_n02_AtbZIP62_LerAmpPoolHmB-B_al2
        31.4%
        41%
        2.2
        HFK3YDRX5_l02_n02_AtbZIP62_ShaAmpPoolHmB-B_al1
        30.3%
        39%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP62_ShaAmpPoolHmB-B_al2
        31.5%
        40%
        2.9
        HFK3YDRX5_l02_n02_AtbZIP72_An1AmpPoolHmB-B_al1
        27.4%
        40%
        2.9
        HFK3YDRX5_l02_n02_AtbZIP72_An1AmpPoolHmB-B_al2
        29.3%
        40%
        4.0
        HFK3YDRX5_l02_n02_AtbZIP72_C24AmpPoolHmB-B_al1
        28.4%
        39%
        4.5
        HFK3YDRX5_l02_n02_AtbZIP72_C24AmpPoolHmB-B_al2
        29.7%
        39%
        6.0
        HFK3YDRX5_l02_n02_AtbZIP72_ColAmpPoolHmB-B_al1
        25.7%
        39%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP72_ColAmpPoolHmB-B_al2
        27.4%
        39%
        4.7
        HFK3YDRX5_l02_n02_AtbZIP72_CviAmpPoolHmB-B_al1
        28.6%
        39%
        3.4
        HFK3YDRX5_l02_n02_AtbZIP72_CviAmpPoolHmB-B_al2
        30.5%
        39%
        4.5
        HFK3YDRX5_l02_n02_AtbZIP72_EriAmpPoolHmB-B_al1
        21.5%
        39%
        2.9
        HFK3YDRX5_l02_n02_AtbZIP72_EriAmpPoolHmB-B_al2
        23.0%
        39%
        3.9
        HFK3YDRX5_l02_n02_AtbZIP72_KyoAmpPoolHmB-B_al1
        25.3%
        39%
        2.5
        HFK3YDRX5_l02_n02_AtbZIP72_KyoAmpPoolHmB-B_al2
        27.1%
        39%
        3.5
        HFK3YDRX5_l02_n02_AtbZIP72_LerAmpPoolHmB-B_al1
        26.2%
        40%
        2.7
        HFK3YDRX5_l02_n02_AtbZIP72_LerAmpPoolHmB-B_al2
        28.0%
        39%
        3.7
        HFK3YDRX5_l02_n02_AtbZIP72_ShaAmpPoolHmB-B_al1
        25.9%
        39%
        4.0
        HFK3YDRX5_l02_n02_AtbZIP72_ShaAmpPoolHmB-B_al2
        27.3%
        39%
        5.3
        HFK3YDRX5_l02_n02_AtbZIP77_An1AmpPoolHmB-B_al1
        30.9%
        41%
        2.9
        HFK3YDRX5_l02_n02_AtbZIP77_An1AmpPoolHmB-B_al2
        31.2%
        42%
        3.1
        HFK3YDRX5_l02_n02_AtbZIP77_C24AmpPoolHmB-B_al1
        31.1%
        40%
        3.7
        HFK3YDRX5_l02_n02_AtbZIP77_C24AmpPoolHmB-B_al2
        31.4%
        41%
        4.0
        HFK3YDRX5_l02_n02_AtbZIP77_ColAmpPoolHmB-B_al1
        28.3%
        40%
        3.1
        HFK3YDRX5_l02_n02_AtbZIP77_ColAmpPoolHmB-B_al2
        28.6%
        41%
        3.1
        HFK3YDRX5_l02_n02_AtbZIP77_CviAmpPoolHmB-B_al1
        31.9%
        40%
        3.2
        HFK3YDRX5_l02_n02_AtbZIP77_CviAmpPoolHmB-B_al2
        32.2%
        41%
        3.3
        HFK3YDRX5_l02_n02_AtbZIP77_EriAmpPoolHmB-B_al1
        23.9%
        40%
        2.5
        HFK3YDRX5_l02_n02_AtbZIP77_EriAmpPoolHmB-B_al2
        24.1%
        40%
        2.5
        HFK3YDRX5_l02_n02_AtbZIP77_KyoAmpPoolHmB-B_al1
        28.5%
        41%
        2.3
        HFK3YDRX5_l02_n02_AtbZIP77_KyoAmpPoolHmB-B_al2
        29.1%
        41%
        2.4
        HFK3YDRX5_l02_n02_AtbZIP77_LerAmpPoolHmB-B_al1
        28.2%
        41%
        2.4
        HFK3YDRX5_l02_n02_AtbZIP77_LerAmpPoolHmB-B_al2
        28.7%
        42%
        2.5
        HFK3YDRX5_l02_n02_AtbZIP77_ShaAmpPoolHmB-B_al1
        29.2%
        40%
        3.4
        HFK3YDRX5_l02_n02_AtbZIP77_ShaAmpPoolHmB-B_al2
        29.6%
        41%
        3.6
        HFK3YDRX5_l02_n02_undetermined
        72.8%
        45%
        51.3

        Demultiplexing Report


        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 129/129 rows and 2/2 columns.
        LibraryTotal Read CountPortion (%)
        undetermined_library
        51270365
        11.1
        AtbZIP34_ColAmpPoolHmB-B_al1
        3508544
        0.8
        AtbZIP34_C24AmpPoolHmB-B_al1
        3567317
        0.8
        AtbZIP34_EriAmpPoolHmB-B_al1
        2895572
        0.6
        AtbZIP34_CviAmpPoolHmB-B_al1
        3463690
        0.7
        AtbZIP34_ShaAmpPoolHmB-B_al1
        3782025
        0.8
        AtbZIP34_KyoAmpPoolHmB-B_al1
        2564418
        0.6
        AtbZIP34_LerAmpPoolHmB-B_al1
        2663017
        0.6
        AtbZIP34_An1AmpPoolHmB-B_al1
        3241036
        0.7
        AtbZIP34_ColAmpPoolHmB-B_al2
        3046076
        0.7
        AtbZIP34_C24AmpPoolHmB-B_al2
        3320307
        0.7
        AtbZIP34_EriAmpPoolHmB-B_al2
        2522584
        0.5
        AtbZIP34_CviAmpPoolHmB-B_al2
        3084015
        0.7
        AtbZIP34_ShaAmpPoolHmB-B_al2
        3354172
        0.7
        AtbZIP34_KyoAmpPoolHmB-B_al2
        2219580
        0.5
        AtbZIP34_LerAmpPoolHmB-B_al2
        2394348
        0.5
        AtbZIP34_An1AmpPoolHmB-B_al2
        2831984
        0.6
        AtbZIP77_ColAmpPoolHmB-B_al1
        3050300
        0.7
        AtbZIP77_C24AmpPoolHmB-B_al1
        3673657
        0.8
        AtbZIP77_EriAmpPoolHmB-B_al1
        2461980
        0.5
        AtbZIP77_CviAmpPoolHmB-B_al1
        3184388
        0.7
        AtbZIP77_ShaAmpPoolHmB-B_al1
        3430293
        0.7
        AtbZIP77_KyoAmpPoolHmB-B_al1
        2319964
        0.5
        AtbZIP77_LerAmpPoolHmB-B_al1
        2402847
        0.5
        AtbZIP77_An1AmpPoolHmB-B_al1
        2946741
        0.6
        AtbZIP77_ColAmpPoolHmB-B_al2
        3100972
        0.7
        AtbZIP77_C24AmpPoolHmB-B_al2
        3963335
        0.9
        AtbZIP77_EriAmpPoolHmB-B_al2
        2501798
        0.5
        AtbZIP77_CviAmpPoolHmB-B_al2
        3281999
        0.7
        AtbZIP77_ShaAmpPoolHmB-B_al2
        3578271
        0.8
        AtbZIP77_KyoAmpPoolHmB-B_al2
        2387739
        0.5
        AtbZIP77_LerAmpPoolHmB-B_al2
        2520798
        0.5
        AtbZIP77_An1AmpPoolHmB-B_al2
        3052174
        0.7
        AtbZIP62_ColAmpPoolHmB-B_al1
        2972999
        0.6
        AtbZIP62_C24AmpPoolHmB-B_al1
        3543482
        0.8
        AtbZIP62_EriAmpPoolHmB-B_al1
        2483143
        0.5
        AtbZIP62_CviAmpPoolHmB-B_al1
        3170463
        0.7
        AtbZIP62_ShaAmpPoolHmB-B_al1
        3493595
        0.8
        AtbZIP62_KyoAmpPoolHmB-B_al1
        2311149
        0.5
        AtbZIP62_LerAmpPoolHmB-B_al1
        2348707
        0.5
        AtbZIP62_An1AmpPoolHmB-B_al1
        2885525
        0.6
        AtbZIP62_ColAmpPoolHmB-B_al2
        2697817
        0.6
        AtbZIP62_C24AmpPoolHmB-B_al2
        2995071
        0.6
        AtbZIP62_EriAmpPoolHmB-B_al2
        2217612
        0.5
        AtbZIP62_CviAmpPoolHmB-B_al2
        2700445
        0.6
        AtbZIP62_ShaAmpPoolHmB-B_al2
        2919894
        0.6
        AtbZIP62_KyoAmpPoolHmB-B_al2
        2064968
        0.4
        AtbZIP62_LerAmpPoolHmB-B_al2
        2153411
        0.5
        AtbZIP62_An1AmpPoolHmB-B_al2
        2546441
        0.6
        AtbZIP54_ColAmpPoolHmB-B_al1
        4157369
        0.9
        AtbZIP54_C24AmpPoolHmB-B_al1
        4199187
        0.9
        AtbZIP54_EriAmpPoolHmB-B_al1
        3387930
        0.7
        AtbZIP54_CviAmpPoolHmB-B_al1
        4141005
        0.9
        AtbZIP54_ShaAmpPoolHmB-B_al1
        4397439
        1.0
        AtbZIP54_KyoAmpPoolHmB-B_al1
        2574116
        0.6
        AtbZIP54_LerAmpPoolHmB-B_al1
        2675400
        0.6
        AtbZIP54_An1AmpPoolHmB-B_al1
        3149325
        0.7
        AtbZIP54_ColAmpPoolHmB-B_al2
        3466887
        0.8
        AtbZIP54_C24AmpPoolHmB-B_al2
        3752671
        0.8
        AtbZIP54_EriAmpPoolHmB-B_al2
        2852413
        0.6
        AtbZIP54_CviAmpPoolHmB-B_al2
        3505966
        0.8
        AtbZIP54_ShaAmpPoolHmB-B_al2
        3806838
        0.8
        AtbZIP54_KyoAmpPoolHmB-B_al2
        2191126
        0.5
        AtbZIP54_LerAmpPoolHmB-B_al2
        2382293
        0.5
        AtbZIP54_An1AmpPoolHmB-B_al2
        2686851
        0.6
        AtbZIP28_ColAmpPoolHmB-B_al1
        3513321
        0.8
        AtbZIP28_C24AmpPoolHmB-B_al1
        4217430
        0.9
        AtbZIP28_EriAmpPoolHmB-B_al1
        2978676
        0.6
        AtbZIP28_CviAmpPoolHmB-B_al1
        3928041
        0.8
        AtbZIP28_ShaAmpPoolHmB-B_al1
        4316346
        0.9
        AtbZIP28_KyoAmpPoolHmB-B_al1
        2658639
        0.6
        AtbZIP28_LerAmpPoolHmB-B_al1
        2768586
        0.6
        AtbZIP28_An1AmpPoolHmB-B_al1
        3447472
        0.7
        AtbZIP28_ColAmpPoolHmB-B_al2
        3274368
        0.7
        AtbZIP28_C24AmpPoolHmB-B_al2
        4297587
        0.9
        AtbZIP28_EriAmpPoolHmB-B_al2
        2741321
        0.6
        AtbZIP28_CviAmpPoolHmB-B_al2
        3784861
        0.8
        AtbZIP28_ShaAmpPoolHmB-B_al2
        4139891
        0.9
        AtbZIP28_KyoAmpPoolHmB-B_al2
        2498350
        0.5
        AtbZIP28_LerAmpPoolHmB-B_al2
        2655829
        0.6
        AtbZIP28_An1AmpPoolHmB-B_al2
        3324705
        0.7
        AtbZIP52_ColAmpPoolHmB-B_al1
        3240916
        0.7
        AtbZIP52_C24AmpPoolHmB-B_al1
        3607269
        0.8
        AtbZIP52_EriAmpPoolHmB-B_al1
        3179152
        0.7
        AtbZIP52_CviAmpPoolHmB-B_al1
        3489537
        0.8
        AtbZIP52_ShaAmpPoolHmB-B_al1
        3423354
        0.7
        AtbZIP52_KyoAmpPoolHmB-B_al1
        2459068
        0.5
        AtbZIP52_LerAmpPoolHmB-B_al1
        2988030
        0.6
        AtbZIP52_An1AmpPoolHmB-B_al1
        4599808
        1.0
        AtbZIP52_ColAmpPoolHmB-B_al2
        2590504
        0.6
        AtbZIP52_C24AmpPoolHmB-B_al2
        3038284
        0.7
        AtbZIP52_EriAmpPoolHmB-B_al2
        2520632
        0.5
        AtbZIP52_CviAmpPoolHmB-B_al2
        2843537
        0.6
        AtbZIP52_ShaAmpPoolHmB-B_al2
        2812041
        0.6
        AtbZIP52_KyoAmpPoolHmB-B_al2
        1943881
        0.4
        AtbZIP52_LerAmpPoolHmB-B_al2
        2424556
        0.5
        AtbZIP52_An1AmpPoolHmB-B_al2
        3645153
        0.8
        AtbZIP51_ColAmpPoolHmB-B_al1
        3386883
        0.7
        AtbZIP51_C24AmpPoolHmB-B_al1
        3670025
        0.8
        AtbZIP51_EriAmpPoolHmB-B_al1
        3301584
        0.7
        AtbZIP51_CviAmpPoolHmB-B_al1
        3449424
        0.7
        AtbZIP51_ShaAmpPoolHmB-B_al1
        3488385
        0.8
        AtbZIP51_KyoAmpPoolHmB-B_al1
        2490585
        0.5
        AtbZIP51_LerAmpPoolHmB-B_al1
        3165953
        0.7
        AtbZIP51_An1AmpPoolHmB-B_al1
        4145915
        0.9
        AtbZIP51_ColAmpPoolHmB-B_al2
        3219534
        0.7
        AtbZIP51_C24AmpPoolHmB-B_al2
        3788217
        0.8
        AtbZIP51_EriAmpPoolHmB-B_al2
        2778378
        0.6
        AtbZIP51_CviAmpPoolHmB-B_al2
        3539646
        0.8
        AtbZIP51_ShaAmpPoolHmB-B_al2
        3846867
        0.8
        AtbZIP51_KyoAmpPoolHmB-B_al2
        2425904
        0.5
        AtbZIP51_LerAmpPoolHmB-B_al2
        2622867
        0.6
        AtbZIP51_An1AmpPoolHmB-B_al2
        3206781
        0.7
        AtbZIP72_ColAmpPoolHmB-B_al1
        3497848
        0.8
        AtbZIP72_C24AmpPoolHmB-B_al1
        4518692
        1.0
        AtbZIP72_EriAmpPoolHmB-B_al1
        2885655
        0.6
        AtbZIP72_CviAmpPoolHmB-B_al1
        3374191
        0.7
        AtbZIP72_ShaAmpPoolHmB-B_al1
        3959454
        0.9
        AtbZIP72_KyoAmpPoolHmB-B_al1
        2545299
        0.6
        AtbZIP72_LerAmpPoolHmB-B_al1
        2749915
        0.6
        AtbZIP72_An1AmpPoolHmB-B_al1
        2934851
        0.6
        AtbZIP72_ColAmpPoolHmB-B_al2
        4686989
        1.0
        AtbZIP72_C24AmpPoolHmB-B_al2
        6005708
        1.3
        AtbZIP72_EriAmpPoolHmB-B_al2
        3909640
        0.8
        AtbZIP72_CviAmpPoolHmB-B_al2
        4541373
        1.0
        AtbZIP72_ShaAmpPoolHmB-B_al2
        5325386
        1.2
        AtbZIP72_KyoAmpPoolHmB-B_al2
        3480120
        0.8
        AtbZIP72_LerAmpPoolHmB-B_al2
        3708177
        0.8
        AtbZIP72_An1AmpPoolHmB-B_al2
        4024128
        0.9

        Barcodes of Undetermined Reads


        We have determined the barcodes of your undetermined reads. Here are the top 20 barcodes. The full list is available here. If your libraries are dual indexed, the two indices are concatenated.

        Showing 20/20 rows and 2/2 columns.
        Barcode Sequence(s)CountFrequency (%)
        GGGGGGGGAGATCTCG
        33235346.0
        64.8
        GGGGGGGGTAACGTCG
        609719.0
        1.2
        GGGGGGGGAACCTCTC
        473290.0
        0.9
        GGGGGGGGCGTGTGAT
        355606.0
        0.7
        GGGGGGGGACGTCCTG
        331591.0
        0.7
        GGGGGGGGTAAGATTA
        320481.0
        0.6
        GGGGGGGGGTCAGTAC
        320376.0
        0.6
        GGGGGGGGCTTCGCCT
        295056.0
        0.6
        GGGGGGGGAGGATAGG
        273151.0
        0.5
        GGGGGGGGGCCTCTAT
        263810.0
        0.5
        GGGGGGGGTCTTACGG
        261636.0
        0.5
        GGGGGGGGTGGAAGCA
        251200.0
        0.5
        GGGGGGGGAACAGGTG
        250151.0
        0.5
        GGGGGGGGAAGGCGTA
        203806.0
        0.4
        GGGGGGGGAGGCTATA
        196523.0
        0.4
        GGGGGGGGAGTTCTCG
        188130.0
        0.4
        ATCGATCGGGGGGGGG
        183080.0
        0.4
        GGGGGGGGCTCGTTCT
        182498.0
        0.4
        TACGCTACGGGGGGGG
        181919.0
        0.3
        GCTATCCTGGGGGGGG
        169727.0
        0.3

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        LaneTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        2.0
        638337024
        462443363
        11.1
        7.2

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (101bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

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