FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26416639
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2133760.8077333380677232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG128900.035.5661965
GCACACG35700.034.45115711
ACGTCTG37950.031.85551515
CTAGTTG82400.031.167378
TACTCTA83800.030.9811824
GCTGACT64200.030.8139151
TCTAGTT86750.030.812747
CTCTAGT86600.030.5044826
TGTACTC84450.030.4817072
GTACTCT84800.030.0182653
TTGTACT83250.029.5874351
CACACGT43850.028.84463512
ACACGTC44850.027.8896713
CACGTCT45000.027.09785314
GGACACC87900.027.07026529
GTTACCT92750.026.5581114
CTAATGC95600.026.53402521
ACCTCTA98600.026.11625517
CTAGCAG81000.026.0981928
TACCTCT103350.024.98355716
Produced by FastQC (version 0.11.5)