FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-9R-Col-0-ABF2-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-9R-Col-0-ABF2-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19867076
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT434320.21861294535743458No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT203490.10242574196625615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG170100.032.615785
GCTGACT105850.032.0424651
CTAGCAG123100.028.0343918
GACTCTA130100.026.9312424
CTAGTTG85500.025.4212998
CTGACTC144300.023.6017342
TACTCTA93350.023.3600234
GGACACC88850.023.08395829
CTCTAGC158600.022.180276
TCTAGTT107650.021.9463337
TAGCAGA159850.021.851849
CTATCGA156550.021.77556217
GACACCT96250.021.3819230
GTACTCT103850.021.3352033
CTCTAGT105200.021.327816
ACCTCTA100350.021.3101917
CTAATGC100150.021.2829621
ACCTACT75450.021.06013733
TCTATCG161650.021.00189216
TGTACTC107800.020.4559292
Produced by FastQC (version 0.11.5)