FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-8R-Col-0-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-8R-Col-0-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23244986
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT580040.2495333832423044No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG326420.14042598261835906No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG298020.12820829403812073No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC285260.1227189381830559No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC271040.11660148988689432No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA257180.1106389136994963No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC236330.10166923740027203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT123200.035.42791
ACTCTAG175450.030.3640255
CTAGCAG156650.028.5994138
CTGACTC158600.027.5863742
GACTCTA164000.027.2972094
CTCTAGC192750.023.752876
TAGCAGA193700.023.2374219
CTATCGA194950.022.67475917
TGACTCT208900.021.698113
TCTATCG203650.021.67171316
GCACACG32700.021.19289411
GATCTAT222600.019.8582414
GCAGATC230000.019.3415211
ATCTATC231150.019.13884715
TAGCATG234700.018.70079627
TATGCCG34000.018.63207846
AGATCTA238750.018.58820713
GTATGCC35550.018.5088745
AGCAGAT246750.018.2839310
CGTATGC36250.018.15145544
Produced by FastQC (version 0.11.5)